Upload and Analyze

Upload .ab1 trace files and guide RNA sequence into the panel on the left. Within seconds you get accurate editing efficiency estimates in addition to qualitative and quantitative information about the indel spectrum. No bioinformatics expertise required!

Use the 'Load example data' feature to see TIDE results on example data.

Use the 'Advanced settings' feature to change TIDE's parameters.

Indel Spectrum

Quality control - Aberrant sequence signal

Remarks


Checklist quality control

Check the following criteria to determine the quality of your data (use the Aberrant sequence signal plot)

  • Is there a considerable divergent signal between control and test sample after the breaksite?
    • Sequences of good quality show:
    • in the control sample (black) a low and equally distributed aberrant sequence signal
    • in the test sample (green) a low signal before the breaksite and a higher signal downstream of the breaksite

  • Is the breaksite at expected location?
    The aberrant sequence signal should increase around the expected cut site (blue dotted line)

  • Does the decomposition window covers a representative sequence?
    For optimal decomposition, the window is set maximal (default), adjust boundaries when the sequence trace is locally of poor quality
If the plot does not meet these criteria, visit the troubleshooting page to see how you can improve your results.

Quantification Indel Frequencies